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Was wondering about this also.
The way I understood it, sex increases testosterone levels (girls can definitely feel if you've been having a lot of sex lately) but fapping decreases it? Is there any biological proof of this?
EDIT: On Day 6 at this moment. Let's see what happens on the famous day 7...

No, no biological proof whatsoever. My question wasn't particularly one I wanted an answer to, as I already knew it. I was just wondering if any of these people who praise the benefits of not fapping had thought it through. Someone above mentions feeling like crap for 3-5 days after a 'cycle' of not fapping. So, presumably, if they had gone a month without masturbating and then got laid, they would have felt like crap for 3-5 days as well, given that you would have been ejaculating the exact same stuff out of your body regardless of whether it was via a girl or your hand?
In which case, it rather begs the question of what is the point in not masturbating to feel better in order to pull more women, only to then feel like shit for 3-5 days after pulling a woman, and having to wait another week until you get back to that level. That means you can have sex with a woman once a fortnight or so?!
So obviously those people having sex more than once a fortnight are feeling like crap constantly and aren't as good at picking up women as those who are only getting sex twice a month, according to the anti-fappers on here.
Either that, or it's actually nothing to do with the biological elements involved whatsoever, and it's all mental; if someone tells you you'll feel better after not masturbating for a month, you trick yourself into actually feeling better after not masturbating for a month. Very similar to the placebo affect.